| 1. | Report of diagnosis and cure on infectious bronchitis
鸡传染性支气管炎的诊治处理报告 |
| 2. | Observed the infectious bronchitis virus shandong isolates by electron microscope
山东省传染性支气管炎病毒分离株的电镜观察 |
| 3. | Isolation and identification of nephropathogenic infectious bronchitis virus in chickens
鸡肾型传染性支气管炎病毒分离及鉴定 |
| 4. | Detection of antibodies against avian infectious bronchitis virus - protocol of agar gel immunodiffusion test
鸡传染性支气管炎抗体检测方法.琼脂免疫扩散试验 |
| 5. | Molecular epidemiology of avian infectious bronchitis viruses isolated in guangxi by pcr and dna sequencing technique
及核苷酸序列分析技术对广西鸡传染性支气管炎病毒进行分子流行病学研究 |
| 6. | Infectious bronchitis ( ib ) caused by infectious bronchitis virus ( ibv ) is an acute and highly contagious disease in chickens
传染性支气管炎( ib )是由传染性支气管炎病毒( ibv )引起的一种急性高度接触性鸡传染病。 |
| 7. | 2 . the potato transformation system that was mediated by agrobacterium fumefaciens ehalos . was established , the optimal callus - forming mediums and callus - differentiating medium were selected and found for potato transformation system . the rates of calli and shoot differentiation were respectively 100 % and more than 90 % for transgenic potato with si gene of infectious bronchitis virus
2建立了马铃薯农杆菌介导的遗传转化体系，确定了马铃薯最佳愈伤诱导培养基及最佳分化培养基，愈伤形成率达100 ，芽的分化率达90以上。 |
| 8. | The sequences of the structural protein genes and deduced a mino acid sequence of isolate lx4 were compared . by computer software , complete main structural genes sequence of ibv domestic strain and molecular characteristic genetic - variant analyses , and probably t cell and b cell epitopes of the main structural protein of infectious bronchitis virus were analyzed premently
通过计算机分析软件，对我国ibv地方流行毒株lx4的主要结构基因全序列、分子特征及遗传变异进行分析比较，并初步分析预测传染性支气管炎病毒主要结构蛋白上可能存在的t细胞和b细胞表位。 |
| 9. | In this paper , a field strain of infectious bronchitis virus was isolated from proventriculus tissue , morphological observation by electron - microscope and the biological characterizations of the virus were studied , pairs of specific primers are designed and synthesized in correspondence with them , according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes , the cdna of si gene , s2 gene , m gene . n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基础上，根据国内外已发表的ibv基因序列，分别设计特异性引物，应用不同引物进行反转录合成cdna ，分片段对ibv的主要结构基因进行pcr扩增，并分别将各个目的片段克隆到puc19载体上，在大肠杆菌dh5中实现目的基因的分子克隆，经蓝白斑筛选、限制性内切酶分析、 pcr鉴定，筛选出重组阳性质粒，并对各个目的基因片段进行序列测定，从而获得ibv主要结构基因全序列。 |
